HDGF Antibody / PWWP Domain Growth Factor Antibody (R32600)

HDGF Antibody in Rat Liver and Human 22Rv1 Prostate Cancer Cells WB. Western blot testing of HDGF Antibody / PWWP Domain Growth Factor Antibody at 0.5 ug/ml in 1) rat liver tissue and 2) human 22Rv1 prostate carcinoma cell lysates. A strong, discrete band is observed at approximately 38 kDa in both samples. Although HDGF has a calculated molecular weight of approximately 27 kDa, endogenous HDGF is well documented to migrate at approximately 37-42 kDa by SDS-PAGE.

HDGF Antibody in Human Lung Carcinoma IHC. Immunohistochemistry testing of HDGF Antibody / PWWP Domain Growth Factor Antibody at 1 ug/ml in FFPE human lung carcinoma tissue. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min. Prominent nuclear immunoreactivity is observed in populations of tumor cells, consistent with the nuclear localization and chromatin-associated functions of HDGF.

HDGF Antibody in Human Intestinal Carcinoma IHC. Immunohistochemistry testing of HDGF Antibody / PWWP Domain Growth Factor Antibody at 1 ug/ml in FFPE human intestinal carcinoma tissue. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min. Nuclear HDGF immunoreactivity is observed in epithelial cells within the tested carcinoma tissue.

HDGF Antibody in Mouse Liver IHC. Immunohistochemistry testing of HDGF Antibody / PWWP Domain Growth Factor Antibody at 1 ug/ml in FFPE mouse liver tissue. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min. Distinct nuclear immunoreactivity is observed in hepatocytes throughout the tested tissue, consistent with the nuclear localization of HDGF.

HDGF Antibody in Rat Liver IHC. Immunohistochemistry testing of HDGF Antibody / PWWP Domain Growth Factor Antibody at 1 ug/ml in FFPE rat liver tissue. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min. Widespread nuclear immunoreactivity is observed in hepatocytes, demonstrating the expected nuclear distribution of HDGF.

HDGF Antibody in Human U-2 OS Osteosarcoma Cells IF. Immunofluorescence testing of FFPE human U-2 OS osteosarcoma cells with HDGF Antibody / PWWP Domain Growth Factor Antibody (green). Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 min. Strong HDGF immunoreactivity is concentrated in the nuclei, consistent with the nuclear localization of this PWWP domain-containing growth factor. Nuclei are counterstained with DAPI (blue).

HDGF Antibody in Human A431 Epidermoid Carcinoma Cells FACS. Flow cytometry testing of human A431 epidermoid carcinoma cells with HDGF Antibody / PWWP Domain Growth Factor Antibody at 1 ug/million cells following blocking with goat serum. Cells alone are shown in red, the isotype control in green and HDGF antibody staining in blue. A clear fluorescence shift is observed relative to both controls, demonstrating detection of HDGF in the tested cells.