HDAC4 Antibody / Histone Deacetylase 4 Antibody (R32738)

HDAC4 Antibody HeLa Cell IF. Immunofluorescence analysis of human HeLa cells using HDAC4 Antibody and an Alpha Tubulin Antibody. Cells underwent enzyme antigen retrieval for 15 minutes, were blocked with 10% goat serum and incubated with both primary antibodies at 5 ug/ml overnight at 4°C. HDAC4 was visualized with Fluoro488-conjugated secondary antibody (green), while alpha tubulin was detected with Cy3-conjugated secondary antibody (red). Strong HDAC4 staining is observed predominantly in the nuclei. This HDAC4 Antibody, also called a Histone Deacetylase 4 Antibody, demonstrates HDAC4 localization in human HeLa cells and supports studies of Class IIa HDAC signaling and transcriptional regulation.

HDAC4 Antibody Human, Mouse and Rat WB. Western blot analysis of HDAC4 in human Jurkat, THP-1, HepG2 and SH-SY5Y cell lysates, rat brain tissue lysate, and mouse brain tissue and NIH3T3 cell lysates using HDAC4 Antibody. Lane 1: Jurkat; Lane 2: THP-1; Lane 3: HepG2; Lane 4: SH-SY5Y; Lane 5: rat brain; Lane 6: mouse brain; Lane 7: NIH3T3. A major band is detected at approximately 140 kDa. Although the calculated molecular weight of HDAC4 is approximately 119 kDa, migration at a higher apparent molecular weight has been reported and can reflect extensive post-translational modification, including phosphorylation. This HDAC4 Antibody, also called a Histone Deacetylase 4 Antibody, detects HDAC4 across human, rat and mouse samples and supports studies of Class IIa HDAC signaling and transcriptional regulation.

HDAC4 Antibody Human Cerebral Cortex IHC. IHC staining of FFPE human cerebral cortex tissue using HDAC4 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive HDAC4 staining is observed in cells within the cerebral cortex. This HDAC4 Antibody, also called a Histone Deacetylase 4 Antibody, demonstrates HDAC4 expression in human brain tissue and supports studies of Class IIa HDAC signaling, neuronal transcription and epigenetic regulation.