HDAC2 Antibody (RQ6487)

IHC staining of FFPE human ovarian cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human gastric cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cervical cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human endometrial cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human gallbladder adenocarcinoma tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse testis tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human Caco-2 cells with HDAC2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human K562, 2) human ThP-1, 3) human HL60, 4) human Caco-2, 5) human HepG2, 6) rat brain, 7) rat lung, 8) rat pancreas, 9) mouse brain and 10) mouse lung lysate with HDAC2 antibody. Predicted molecular weight: 55-60 kDa.

Flow cytometry testing of human HL60 cells with HDAC2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HDAC2 antibody.