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Home >> Antibodies >> HDAC1 Antibody

HDAC1 Antibody [clone PCRP-HDAC1-1B7] (V9651)

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  Catalog No Formulation Size Price (USD)  
Image V9651-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9651-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9651SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HDAC1 antibody (clone PCRP-HDAC1-1B7). These results demonstrate the foremost specificity of the PCRP-HDAC1-1B7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HDAC1 antibody (clone PCRP-HDAC1-1B7). These results demonstrate the foremost specificity of the PCRP-HDAC1-1B7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Immunofluorescent staining of PFA-fixed human HeLa cells using HDAC1 antibody (green, clone PCRP-HDAC1-1B7) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with HDAC1 antibody (blue, clone PCRP-HDAC1-1B7) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free HDAC1 antibody (clone PCRP-HDAC1-1B7) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG
Clone Name PCRP-HDAC1-1B7
Purity Protein A/G affinity
UniProt Q13547
Applications ELISA (order BSA-free format for coating) :
Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Limitations This HDAC1 antibody is available for research use only.
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Description

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/ CBP-associated factor), p300/CBP and the TFIID subunit TAFII p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Application Notes

Optimal dilution of the HDAC1 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human protein was used as the immunogen for the HDAC1 antibody.

Storage

Aliquot the HDAC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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