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Home >> Antibodies >> HCAM Antibody for IF / Cell Surface Localization Antibody

HCAM Antibody for IF / Cell Surface Localization Antibody [clone 7H7] (RQ6270)

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Image RQ6270 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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HCAM Antibody A431 Cell IF. Immunofluorescence analysis of HCAM / CD44 expression in human A431 cells using mouse monoclonal antibody clone 7H7 (green) with DAPI nuclear stain (blue). Strong membrane-associated fluorescence is observed outlining cell borders, highlighting cell surface localization consistent with HCAM/CD44 expression. The staining pattern demonstrates clear cell perimeter labeling and supports its use for visualizing receptor distribution and cellular morphology in cultured epithelial cells.
HCAM Antibody Human and Rat Sample WB. Western blot testing of 1) human HeLa, 2) human A549 and 3) rat C6 cell lysate with HCAM antibody. Predicted molecular weight ~81 kDa.
HCAM Antibody Human Sample FACS. Flow cytometry testing of human U-87 MG cells with HCAM antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HCAM antibody.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 7H7
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P16070
Localization Cell membrane, cytoplasm
Applications Western Blot : 1-2ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Limitations This HCAM antibody is available for research use only.
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Description

Homing cell adhesion molecule (HCAM), also known as CD44 antigen, is a transmembrane glycoprotein that functions as a receptor for hyaluronic acid and mediates cell adhesion, migration, and extracellular matrix interactions. It is expressed on a wide range of cell types, where it contributes to cell surface communication and interaction with the extracellular environment. HCAM Antibody for IF / Cell Surface Localization Antibody is designed to detect HCAM/CD44 expression in immunofluorescence applications, enabling high-resolution visualization of membrane-associated localization in cultured cell systems.

HCAM antibody, also referred to as CD44 antibody or Hermes antigen antibody, recognizes a cell surface glycoprotein involved in adhesion and extracellular matrix interaction. Detection in cultured cell lines such as A431 provides a controlled system for evaluating receptor distribution, membrane localization, and cellular morphology. Immunofluorescence imaging allows clear delineation of cell boundaries and assessment of subcellular localization patterns.

Functionally, HCAM/CD44 mediates interactions with hyaluronic acid and other extracellular matrix components, supporting cell adhesion and spatial organization at the plasma membrane. In immunofluorescence applications, staining typically appears as strong membrane-associated fluorescence outlining cell borders, with minimal nuclear signal and limited cytoplasmic diffusion. This HCAM Antibody for IF is particularly suited for examining cell surface distribution, receptor localization, and cell morphology in epithelial cell models.

HCAM/CD44 expression in A431 cells provides a robust system for analyzing membrane-associated protein localization and receptor organization. Detection in this context supports studies of cell surface signaling, adhesion dynamics, and spatial distribution of CD44 within cultured cells. The use of a mouse monoclonal antibody clone 7H7 provides consistent and specific detection of HCAM/CD44 in immunofluorescence assays.

Structurally, HCAM/CD44 consists of an extracellular ligand-binding domain, a transmembrane segment, and a cytoplasmic tail involved in intracellular signaling. Alternative splicing generates multiple isoforms, while glycosylation contributes to structural variation. An antibody targeting HCAM/CD44 is suitable for detecting membrane-associated expression and studying cell surface localization in immunofluorescence and imaging-based applications.

This CD44 antibody is part of a broader CD44 antibody panel offered by NSJ Bioreagents.

Application Notes

Optimal dilution of the HCAM Antibody for IF / Cell Surface Localization Antibody should be determined by the researcher.

Immunogen

Amino acids RFAGVFHVEKNGRYSISRTEAADLCKAF from the human protein were used as the immunogen for the HCAM antibody.

Storage

After reconstitution, the HCAM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

HCAM antibody, CD44 antibody, HCAM immunofluorescence antibody, CD44 cell surface antibody, Hermes antigen antibody

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