HCAM Antibody Clone 156-3C11 / CD44 Tumor Biology Research Antibody [clone 156-3C11] (V2068)

Description

Homing cell adhesion molecule (HCAM), also known as CD44 antigen, is a transmembrane glycoprotein that functions as a receptor for hyaluronic acid and mediates cell adhesion, migration, and extracellular matrix interactions. It is expressed on the surface of epithelial cells, immune cells, and tumor cells, where it plays a central role in cell-cell communication and interaction with the surrounding microenvironment. HCAM Antibody Clone 156-3C11 / CD44 Tumor Biology Research Antibody is designed to detect HCAM/CD44 expression in formalin-fixed, paraffin-embedded tissues and other research samples, enabling immunohistochemistry-based evaluation of cell surface localization, tumor cell organization, and microenvironment-associated expression patterns in cancer research settings.

HCAM antibody, also referred to as CD44 antibody or Hermes antigen antibody, recognizes a cell surface glycoprotein involved in adhesion and extracellular matrix engagement. Clone 156-3C11 is a mouse monoclonal antibody that has been referenced in numerous peer-reviewed studies, particularly in tumor biology and cancer research. Its frequent use in these contexts supports its role as a well-established reagent for investigating HCAM/CD44 expression in malignant cells and their interaction with stromal and immune components within the tumor microenvironment.

Functionally, HCAM/CD44 mediates binding to hyaluronic acid and other extracellular matrix components, facilitating tumor cell adhesion, migration, and spatial organization within tissues. These interactions are especially relevant at the cell surface, where HCAM/CD44 supports communication between tumor cells and the surrounding extracellular matrix. In immunohistochemistry applications, staining typically presents as strong membranous HRP-DAB signal outlining tumor cell borders, reflecting its localization at the plasma membrane. This HCAM Antibody Clone 156-3C11 is particularly suited for examining tumor cell morphology, architectural organization, and microenvironmental interactions in carcinoma samples.

HCAM/CD44 expression is observed across a wide range of tumor types, where it contributes to cellular heterogeneity, organization of tumor cell clusters, and interaction with stromal elements. Variability in staining intensity and distribution may reflect differences in tumor differentiation state, cellular organization, and microenvironmental context. Detection of HCAM/CD44 therefore supports detailed evaluation of tumor biology, including cell surface interaction dynamics and structural organization within malignant tissues. The citation history of clone 156-3C11 further supports its use as a reliable and frequently utilized reagent in cancer-focused experimental systems.

Structurally, HCAM/CD44 is encoded on chromosome 11p13 and consists of an extracellular ligand-binding domain, a transmembrane segment, and a cytoplasmic tail involved in intracellular signaling and cytoskeletal interactions. Alternative splicing generates multiple CD44 isoforms that retain core functional properties while contributing to biological diversity. An antibody targeting HCAM/CD44 is suitable for detecting membrane-associated expression and studying cell surface interactions, tumor architecture, and microenvironmental communication in cancer biology and related research applications.

This CD44 antibody is part of a broader CD44 antibody panel offered by NSJ Bioreagents.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCAM Antibody Clone 156-3C11 / CD44 Tumor Biology Research Antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.

Storage

Store the HCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

HCAM antibody, CD44 antibody, HCAM 156-3C11 antibody, CD44 antigen antibody, Hermes antigen antibody