HBEGF Antibody / Heparin-Binding EGFR Ligand and Epithelial Signaling Marker [clone HBEGF/9503] (V5687)

HBEGF Antibody RAW264.7 WB. Western blot analysis of mouse RAW264.7 cell lysate using HBEGF antibody detecting Heparin-binding EGF-like growth factor (HB-EGF), clone HBEGF/9503. A band is observed at approximately 25-30 kDa, consistent with the predicted molecular weight of HBEGF, with possible upward shift reflecting glycosylation and processing of the membrane-associated precursor protein (proHB-EGF). This pattern aligns with the known biology of HBEGF as a heparin-binding EGFR ligand that undergoes proteolytic shedding to generate soluble signaling forms.

HBEGF Antibody Skin IHC. Immunohistochemistry analysis of FFPE human skin tissue stained with HBEGF antibody detecting Heparin-binding EGF-like growth factor (HB-EGF), clone HBEGF/9503. Epidermal keratinocytes show membranous and cytoplasmic staining, consistent with expression of proHB-EGF at the cell surface and its role as a heparin-binding EGFR ligand involved in epithelial signaling and tissue repair. The staining pattern highlights epithelial cell layers, while underlying dermal components show comparatively lower signal. The inset shows a PBS-only negative control processed without primary antibody, confirming minimal non-specific background staining. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free HBEGF antibody (clone HBEGF/9503) as confirmation of integrity and purity.

HBEGF Antibody Heart and PANC28 WB. Western blot analysis of human heart and PANC28 cell lysates using HBEGF antibody detecting Heparin-binding EGF-like growth factor (HB-EGF), clone HBEGF/9503. A band is detected at approximately 25-30 kDa in both samples, consistent with the predicted molecular weight of HBEGF, with size reflecting glycosylation and processing of the membrane-associated precursor (proHB-EGF). This pattern aligns with the biology of HBEGF as a heparin-binding EGFR ligand that undergoes regulated shedding to generate soluble signaling forms in both normal tissue and carcinoma-derived cells.