HBEGF Antibody / DT-R - Diphtheria Toxin Receptor and EGFR Ligand Marker (RQ7309)

HBEGF Antibody Colorectal Adenocarcinoma IF. Immunofluorescence analysis of FFPE human colorectal adenocarcinoma tissue stained with HBEGF antibody (red) detecting Heparin-binding EGF-like growth factor (DT-R). Tumor epithelial structures show prominent membranous and cytoplasmic fluorescence, consistent with localization of HBEGF as a cell surface receptor capable of mediating diphtheria toxin binding and internalization, as well as EGFR ligand signaling. The staining pattern highlights carcinoma glands with enriched signal along epithelial cell borders. DAPI nuclear stain (blue) highlights nuclei. HIER: steam section in pH 8 EDTA buffer for 20 min.

HBEGF Antibody Appendiceal Adenocarcinoma IHC. Immunohistochemistry analysis of FFPE human appendiceal adenocarcinoma tissue stained with HBEGF antibody detecting Heparin-binding EGF-like growth factor (DT-R). Tumor epithelial cells show membranous and cytoplasmic staining, consistent with localization of HBEGF as a cell surface receptor involved in diphtheria toxin binding and receptor-mediated internalization, as well as EGFR ligand signaling. The staining outlines glandular tumor structures, while surrounding stromal components show comparatively reduced signal. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

HBEGF Antibody Lung Adenocarcinoma IHC. Immunohistochemistry analysis of FFPE human lung adenocarcinoma tissue stained with HBEGF antibody detecting Heparin-binding EGF-like growth factor (DT-R). Tumor epithelial cells show membranous and cytoplasmic staining, consistent with localization of HBEGF as a cell surface receptor involved in diphtheria toxin binding and receptor-mediated internalization, as well as EGFR ligand signaling. The staining highlights glandular tumor architecture, while surrounding stromal elements show comparatively reduced signal. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

HBEGF Antibody Mouse Lung IHC. Immunohistochemistry analysis of FFPE mouse lung tissue stained with HBEGF antibody detecting Heparin-binding EGF-like growth factor (DT-R). Epithelial cells lining airway and alveolar structures show membranous and cytoplasmic staining, consistent with localization of HBEGF as a cell surface receptor capable of mediating diphtheria toxin binding and receptor-mediated internalization, as well as EGFR ligand signaling. Surrounding stromal regions show comparatively lower signal. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

HBEGF Antibody Rat Respiratory Smooth Muscle IHC. Immunohistochemistry analysis of FFPE rat respiratory smooth muscle tissue stained with HBEGF antibody detecting Heparin-binding EGF-like growth factor (DT-R). Smooth muscle cells show cytoplasmic and membranous staining, consistent with expression of HBEGF as a membrane-associated precursor that can function as a diphtheria toxin receptor and EGFR ligand. Surrounding connective tissue shows comparatively reduced signal. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

HBEGF Antibody Multi-Sample WB. Western blot analysis of human, rat, and mouse lysates using HBEGF antibody detecting Heparin-binding EGF-like growth factor (DT-R). Lane 1: HeLa, Lane 2: K562, Lane 3: Jurkat, Lane 4: U-251, Lane 5: rat C6, Lane 6: mouse lung tissue. A band is detected at approximately 23-30 kDa across multiple samples, consistent with the predicted molecular weight of HBEGF, with variation reflecting glycosylation and processing of the membrane-associated precursor (proHB-EGF). The detection pattern aligns with the known biology of HBEGF as a cell surface receptor mediating diphtheria toxin binding and internalization, as well as an EGFR ligand involved in growth factor signaling across diverse cell types.

HBEGF Antibody Jurkat FACS. Flow cytometry analysis of fixed human Jurkat cells stained with HBEGF antibody detecting Heparin-binding EGF-like growth factor (DT-R). The antibody signal (blue) shows a clear rightward shift compared to cells alone (red) and isotype control (green), indicating positive detection of HBEGF. This profile is consistent with HBEGF as a cell surface-associated receptor capable of mediating diphtheria toxin binding and internalization, as well as functioning as an EGFR ligand in signaling pathways.