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Home >> Antibodies >> HAUS7 Antibody / HAUS augmin-like complex subunit 7 / UCHL5IP

HAUS7 Antibody / HAUS augmin-like complex subunit 7 / UCHL5IP (RQ8726)

  Catalog No Formulation Size Price (USD)  
Image RQ8726 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human A549 cells with HAUS7 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) MCF7 and 2) A549 cell lysate with HAUS7 antibody. Predicted molecular weight ~40 kDa.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with HAUS7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HAUS7 antibody.
Availability 1-3 days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q99871
Localization Cytoplasm
Applications Western Blot : 1-2ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This HAUS7 antibody is available for research use only.
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Description

This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the HAUS7 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids D18-Q333) was used as the immunogen for the HAUS7 antibody.

Storage

After reconstitution, the HAUS7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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