HAUS6 Antibody / HAUS augmin-like complex subunit 6 / FAM29A (FY13188)

Immunofluorescent staining of FAM29A/HAUS6 using anti-HAUS6 antibody (green) and anti-Beta Tubulin antibody (red). FAM29A/HAUS6 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-HAUS6 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and DyLight 594 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Western blot analysis of FAM29A/HAUS6 using anti-HAUS6 antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAUS6 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for FAM29A/HAUS6 at approximately 109 kDa. The expected molecular weight of FAM29A/HAUS6 is at 109 kDa.

Immunoprecipitating FAM29A/HAUS6 in MCF-7 whole cell lysate. Western blot analysis of FAM29A/HAUS6 using anti-HAUS6 antibody; Lane 1: MCF-7 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-HAUS6 antibody in MCF-7 whole cell lysate; Lane 3: anti-HAUS6 antibody (2ug) + MCF-7 whole cell lysate (500ug). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HAUS6 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for FAM29A/HAUS6 at approximately 109 kDa. The expected molecular weight of FAM29A/HAUS6 is at 109 kDa.