Haptoglobin Antibody HP/3835 / Haptoglobin Protein Antibody [clone HP/3835] (V8846)

Haptoglobin Antibody HP/3835 Staining of Human Liver IHC. Immunohistochemistry testing of FFPE human liver tissue with Haptoglobin Antibody clone HP/3835 at 2 ug/ml shows strong brown cytoplasmic staining throughout hepatocytes. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before antibody testing.

Haptoglobin Protein Antibody HP/3835 Staining of Human Liver IHC. Immunohistochemistry testing of FFPE human liver tissue with Haptoglobin Protein Antibody clone HP/3835 at 2 ug/ml shows widespread granular brown cytoplasmic staining of hepatocytes, with nuclei remaining predominantly counterstained blue. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before antibody testing.

Purified Haptoglobin Antibody HP/3835 SDS-PAGE. SDS-PAGE analysis of 2 ug purified, BSA-free Haptoglobin Antibody clone HP/3835 under non-reducing (NR) and reducing (R) conditions. The non-reduced antibody migrates at approximately 150 kDa, while reduction produces the expected immunoglobulin heavy and light chain bands at approximately 50 and 25 kDa, respectively, supporting antibody integrity and purity.

Protein Microarray Validation of Haptoglobin Antibody HP/3835. Protein microarray analysis of Haptoglobin Antibody clone HP/3835 using a HuProt array containing more than 19,000 full-length human proteins identifies HP as the dominant binding target. HP ranks first with a Z-score of approximately 123.9 and an S-score of approximately 121.8, showing exceptionally strong separation from the next-highest array signal. The Z-score represents binding signal relative to the mean signal across the array, while the S-score represents separation between the intended target and the next-highest protein signal.

Haptoglobin Antibody HP/3835 Detection in Human Liver WB. Western blot analysis of human liver tissue lysate using Haptoglobin Antibody clone HP/3835. A strong, discrete band is detected at approximately 24 kDa. Haptoglobin is synthesized as an approximately 45 kDa precursor that undergoes proteolytic processing into alpha and beta chains; glycosylation of the processed protein can influence its apparent molecular weight on SDS-PAGE.