HAAO Antibody / 3HAO Antibody (RQ8821)

HAAO Antibody staining of human liver cancer by IHC. Immunohistochemistry of FFPE human liver cancer sections using the HAAO Antibody at 2 ug/ml. Strong brown staining is broadly distributed throughout the cytoplasm of hepatocellular tumor cells, while nuclei are counterstained blue and remain predominantly negative. The staining is relatively widespread but varies in intensity among individual cells, producing a predominantly cytoplasmic pattern consistent with the reported localization of HAAO. Heat-mediated antigen retrieval was performed in pH 8 EDTA before overnight primary antibody incubation at 4oC. The staining demonstrates tissue detection of 3-hydroxyanthranilate 3,4-dioxygenase with this 3HAO Antibody.

HAAO Antibody detection across human, rat and mouse samples by WB. Western blot testing with the HAAO Antibody under reducing conditions. Lane 1: human HepG2; lane 2: human THP-1; lane 3: human HCCP; lane 4: rat liver; lane 5: mouse liver. Thirty micrograms of protein were loaded per lane. A specific band is detected at approximately 33 kDa across all samples, matching the calculated molecular weight of 32.6 kDa. Rat and mouse liver show particularly strong signal, while the HCCP lane also displays higher-molecular-weight background. The results demonstrate cross-species detection of HAAO with this 3HAO Antibody.