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Home >> Antibodies >> HA Tag Antibody

HA Tag Antibody [clone HA/279] (V8914)

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  Catalog No Formulation Size Price (USD)  
Image V8914-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8914-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8914SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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SDS-PAGE analysis of purified, BSA-free HA Tag antibody (clone HA/279) as confirmation of integrity and purity.
SDS-PAGE analysis of purified, BSA-free HA Tag antibody (clone HA/279) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity HA tagged proteins
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name HA/279
Purity Protein A/G affinity
Applications ELISA (order BSA-free format for coating) :
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunoprecipitation : 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Flow cytometry : 1-2ug/million cells
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This HA Tag antibody is available for research use only.
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Description

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Application Notes

Optimal dilution of the HA Tag antibody should be determined by the researcher.

Immunogen

Amino acids 98-106 (YPYDVPDYA) were used as the immunogen for the HA Tag antibody.

Storage

Aliquot the HA Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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