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Home >> Antibodies >> HA Antibody

HA Antibody (F52062)

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  Catalog No Formulation Size Price (USD)  
Image F52062-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F52062-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Western blot analysis of lysate from 12 tagged recombinant protein cell lines using HA antibody diluted at 1:1000.
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Western blot analysis of lysate from 12 tagged recombinant protein cell lines using HA antibody diluted at 1:1000.
Western blot analysis of HA antibody and tagged recombinant protein bacterial lysate
GTPase activity of Dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient Dynamin-2 mutant with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko)
Availability 1-3 business days
Species Reactivity HA tag
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
ChIP : 1:100
Immunoprecipitation : suitable
Limitations This HA antibody is available for research use only.
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Description

Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. NSJBio's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.

Application Notes

Titration of the HA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

The sequence CYPYDVPDYAYPYDVPDYA was used as the immunogen for this HA antibody.

Storage

Aliquot the HA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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