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Home >> Antibodies >> H3K4me3 Antibody / Trimethyl-Histone H3 (Lys4)

H3K4me3 Antibody / Trimethyl-Histone H3 (Lys4) [clone RM340] (R20363)

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  Catalog No Formulation Size Price (USD)  
Image R20363-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Microvalidated Recrabbitmono
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SNAP-ChIP (TM) / qPCR using anti-H3K4me3 antibody. Antibody (3 ug at 17 ug/ml) was tested in native ChIP with 3 ug HEK-293 chromatin (~1x10e6 cells). Specificity (left Y-axis; all bars mean ± SEM from six independent ChIP experiments; note less than 15% cross-reactivity outside H3K4me3) was determined by quantitative real-time PCR (qPCR) for the duplicate DNA barcodes corresponding to each modified nucleosome in the SNAP-ChIP K-MetStat panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input.
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SNAP-ChIP (TM) / qPCR using anti-H3K4me3 antibody. Antibody (3 ug at 17 ug/ml) was tested in native ChIP with 3 ug HEK-293 chromatin (~1x10e6 cells). Specificity (left Y-axis; all bars mean ± SEM from six independent ChIP experiments; note less than 15% cross-reactivity outside H3K4me3) was determined by quantitative real-time PCR (qPCR) for the duplicate DNA barcodes corresponding to each modified nucleosome in the SNAP-ChIP K-MetStat panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input.
ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with H3 K4 Modified), using anti-H3K4me3 antibody (10 ug/ml).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM340
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Applications Chromatin IP : 1ug/ml-5ug/ml
ELISA : 1ug/ml-10ug/ml
Limitations This recombinant H3K4me3 antibody is available for research use only.
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Description

This antibody reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me3), or other methylation in histone H3.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K4me3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4) was used as the immunogen for the recombinant H3K4me3 antibody.

Storage

Store the recombinant H3K4me3 antibody at -20oC.

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