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Home >> Antibodies >> Gusb Antibody / Beta Glucuronidase

Gusb Antibody / Beta Glucuronidase (RQ5612)

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  Catalog No Formulation Size Price (USD)  
Image RQ5612 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat liver and 2) mouse liver lysate with Gusb antibody. Expected molecular weight: 68-75 kDa, also seen as dimers and tetramers.
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Western blot testing of 1) rat liver and 2) mouse liver lysate with Gusb antibody. Expected molecular weight: 68-75 kDa, also seen as dimers and tetramers.
IHC staining of FFPE mouse liver with Gusb antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver with Gusb antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney with Gusb antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney with Gusb antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P12265
Applications Western blot : 0.25-0.5ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Gusb antibody is available for research use only.
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Description

Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.

Application Notes

Optimal dilution of the Gusb antibody should be determined by the researcher.

Immunogen

A mouse recombinant protein (amino acids R122-E575) was used as the immunogen for the Gusb antibody.

Storage

After reconstitution, the Gusb antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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