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Home >> Antibodies >> GST Tag Antibody / HRP Conjugate

GST Tag Antibody / HRP Conjugate [clone DH-7] (RQ4677)

  Catalog No Formulation Size Price (USD)  
Image RQ4677 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
Microvalidated Rab Mono Image
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Western blot testing of recombinant GST with GST Tag antibody. Expected molecular weight of GST Tag ~26 kDa.
Availability 1-2 weeks
Species Reactivity GST Tag
Format HRP Conjugate
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name DH-7
Purity Affinity purified
Applications Western blot : 1:3000-1:10000
Limitations This GST Tag antibody is available for research use only.
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Description

Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.

Application Notes

Optimal dilution of the GST Tag antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to GST was used as the immunogen for the GST Tag antibody.

Storage

Store the GST Tag antibody at -20oC.

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