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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> GST Antibody

GST Antibody [clone 21CT54.13.1] (F52061)

  Catalog No Formulation Size Price (USD)  
Image F52061-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F52061-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of GST antibody and recombinant protein.
Availability 1-3 business days
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, k
Clone Name 21CT54.13.1
Purity Purified
Applications Western blot : 1:100-1:500
Limitations This GST antibody is available for research use only.
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Description

Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.

Application Notes

Titration of the GST antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Purified recombinant fusion protein was used to produced this monoclonal GST antibody.

Storage

Aliquot the GST antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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