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Home >> Antibodies >> GSDMD Antibody / Gasdermin D

GSDMD Antibody / Gasdermin D [clone 6D11] (RQ7955)

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  Catalog No Formulation Size Price (USD)  
Image RQ7955 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human PC-3 cells with GSDMD antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human PC-3 cells with GSDMD antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human esophageal squamous carcinoma tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical intraepithelial neoplasia tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) ThP-1, 2) SW620, 3) Jurkat, 4) U-87 MG and 5) PC-3 cell lysate with GSDMD antibody. Predicted molecular weight ~53 kDa.
Flow cytometry testing of human Jurkat cells with GSDMD antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GSDMD antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 6D11
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P57764
Localization Nucleus, cytoplasm, cell membrane, secreted
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This GSDMD antibody is available for research use only.
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Description

Gasdermin D is a precursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals. [UniProt]

Application Notes

Optimal dilution of the GSDMD antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-H484) was used as the immunogen for the GSDMD antibody.

Storage

After reconstitution, the GSDMD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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