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Home >> Antibodies >> GRP78 BiP Antibody / HSPA5

GRP78 BiP Antibody / HSPA5 [clone AII-8] (RQ5349)

  Catalog No Formulation Size Price (USD)  
Image RQ5349 Antibody in PBS with 0.02% sodium azide and 50% glycerol 100 ul 429
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IHC staining of FFPE human testis tissue with GRP78 BiP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of 1) human HeLa and 2) mouse C6 cell lysate with GRP78 BiP antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
Availability 1-2 weeks
Species Reactivity Human, Rat
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name AII-8
Purity Affinity purified
UniProt P11021
Localization Cytoplasmic
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This GRP78 BiP antibody is available for research use only.
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Description

The protein encoded by the HSPA5 gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell. [RefSeq]

Application Notes

Optimal dilution of the GRP78 BiP antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human GRP78 / BiP / HSPA5 was used as the immunogen for the GRP78 BiP antibody.

Storage

Store the GRP78 BiP antibody at -20oC.

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