GRP78 Antibody / Endoplasmic Reticulum Chaperone Antibody (R31939)

GRP78 Antibody HeLa Cell IF. GRP78 was detected in HeLa cells following enzyme-mediated antigen retrieval for 15 minutes. The cells were blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-HSPA5 antibody at 5 ug/ml. A Fluoro488-conjugated goat anti-rabbit IgG secondary antibody was used at 1:500, and nuclei were counterstained with DAPI. GRP78 staining appears green and is distributed throughout the cytoplasm, including prominent perinuclear signal, while nuclei appear blue. GRP78 Antibody, an endoplasmic reticulum chaperone antibody, supports cellular localization studies involving protein folding, endoplasmic reticulum homeostasis and stress-response pathways.

GRP78 Antibody Human Cerebral Cortex IHC. GRP78 was detected in a paraffin-embedded section of human cerebral cortex tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-HSPA5 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining demonstrates broadly distributed GRP78 expression within the cerebral cortex, including cytoplasmic signal in neuronal cells and surrounding neuropil. GRP78 Antibody, an endoplasmic reticulum chaperone antibody, supports studies of protein-folding homeostasis and cellular stress responses in human brain tissue.

GRP78 Antibody Mouse Testis IHC. GRP78 was detected in a paraffin-embedded section of mouse testis tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-HSPA5 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. Cytoplasmic GRP78 staining is visible within the seminiferous tubules, with prominent signal among populations of developing germ cells and weaker staining in the interstitial tissue. GRP78 Antibody, an endoplasmic reticulum chaperone antibody, supports studies of protein folding and cellular stress responses in male reproductive tissue.

GRP78 Antibody Rat Testis Germ Cell IHC. GRP78 expression was examined in a paraffin-embedded section of rat testis tissue. Heat-mediated antigen retrieval was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The section was incubated overnight at 4°C with rabbit anti-HSPA5 antibody at 2 ug/ml, then developed using an HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen. Strong cytoplasmic staining is visible among developing germ cell populations within the seminiferous tubules. GRP78 Antibody, an endoplasmic reticulum chaperone antibody, supports studies of protein folding and cellular stress regulation in rat reproductive tissue.

GRP78 Antibody Human, Rat and Mouse Cell and Tissue WB. Western blot analysis was performed under reducing conditions using 30 ug of lysate per lane. Lane 1: human U251 cells; Lane 2: human HepG2 cells; Lane 3: human K562 cells; Lane 4: human A431 cells; Lane 5: rat testis; Lane 6: rat liver; Lane 7: mouse testis; Lane 8: mouse liver. The membrane was incubated overnight at 4°C with rabbit anti-HSPA5 antibody at 0.5 ug/ml, followed by HRP secondary antibody and ECL detection. A prominent band was detected at approximately 78 kDa, near the expected 72 kDa size. GRP78 Antibody, an endoplasmic reticulum chaperone antibody, supports multi-species expression analysis.