GRID1 Antibody / Glutamate receptor ionotropic, delta-1 Antibody (FY13546)

GRID1 Antibody by IHC in human glioma tissue. Immunohistochemistry of paraffin-embedded human glioma using Glutamate receptor ionotropic, delta-1 Antibody demonstrates brown immunoreactivity among tumor cells, with heterogeneous staining distributed throughout the examined field. Blue hematoxylin counterstaining distinguishes cell nuclei from the surrounding immunoreactive regions. Tissue sections underwent heat-mediated antigen retrieval in EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, and bound antibody was detected using an HRP-conjugated secondary antibody and DAB chromogen. The image documents GRID1-associated immunoreactivity in human glioma.

GRID1 Antibody by WB in human and rat cell lysates. Western blot analysis using Glutamate receptor ionotropic, delta-1 Antibody detects a principal band near 112 kDa, consistent with the calculated GRID1 molecular weight. Lanes 1-4 contain human HEL, K562, SH-SY5Y and U2OS whole-cell lysates, respectively; lane 5 contains rat C6 cell lysate. Reduced samples containing 30 ug protein per lane were resolved by 8% SDS-PAGE and transferred to nitrocellulose. Membranes were incubated with 0.5 ug/mL primary antibody overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection. The labeled band migrates at a comparable position across the five samples.

GRID1 Antibody by FACS in SH-SY5Y cells. Flow cytometry analysis using Glutamate receptor ionotropic, delta-1 Antibody shows a blue antibody-stained fluorescence distribution shifted to the right of the red unstained control, with the green isotype control also displayed for comparison. SH-SY5Y cells were fixed in 4% paraformaldehyde, blocked with normal goat serum and incubated with 1 ug primary antibody per 1 x 10^6 cells for 30 minutes at 20oC. A fluorescent anti-rabbit secondary antibody was applied for an additional 30 minutes. The histogram illustrates GRID1-associated staining relative to the control populations under these assay conditions.