GRIA4 Antibody / Glutamate Receptor 4 Antibody (R34495)

GRIA4 Antibody Human Cerebellum WB. Western blot analysis was performed on human cerebellum lysate using GRIA4 Antibody at 0.3 ug/ml. A specific immunoreactive band is detected at approximately 97–101 kDa, consistent with the expected molecular weight of GRIA4. The slight difference in apparent migration is typical of large membrane-associated glutamate receptor subunits and may reflect post-translational modification or gel migration characteristics. These results demonstrate that this Glutamate Receptor 4 Antibody is suitable for Western blot detection of endogenous GRIA4 in human cerebellum tissue.

GRIA4 Antibody Human Cortex IHC. Immunohistochemistry was performed on FFPE human cerebral cortex tissue using GRIA4 Antibody at 5 ug/ml. Heat-induced epitope retrieval was carried out in pH 6 citrate buffer, and antibody binding was visualized using an alkaline phosphatase detection system. Distinct cytoplasmic staining is observed in cortical neurons, while the surrounding neuropil exhibits weaker immunoreactivity, consistent with the neuronal expression pattern of GRIA4 as an AMPA receptor subunit. These results demonstrate that this Glutamate Receptor 4 Antibody is suitable for immunohistochemical detection of GRIA4 in formalin-fixed, paraffin-embedded human brain tissue.