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Home >> Antibodies >> Granzyme B Antibody

Granzyme B Antibody (RQ4165)

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Image RQ4165 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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Granzyme B Antibody Mouse Thymus and Liver WB. Western blot analysis of mouse thymus and liver tissue lysates using Granzyme B Antibody at 0.5 ug/ml. An HRP-conjugated secondary antibody with ECL substrate was used for detection. Granzyme B Antibody, a Cytotoxic Lymphocyte Marker Antibody, detects bands within the expected 29-37 kDa molecular weight range, consistent with precursor and proteolytically processed forms of granzyme B. The higher molecular weight band likely represents the inactive proenzyme, while the lower band corresponds to the mature active enzyme.
Availability 1-3 business days
Species Reactivity Mouse
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P04187
Applications Western Blot : 0.5-1ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Granzyme B antibody is available for research use only.
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Description

Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

Learn more about our Granzyme B Antibody, a Cytotoxic Lymphocyte Marker Antibody for investigating immune cell activation, cell-mediated cytotoxicity, tumor immunity, and natural killer cell function.

Application Notes

Optimal dilution of the Granzyme B antibody should be determined by the researcher.

Immunogen

A recombinant mouse protein corresponding to amino acids I21-S247 was used as the immunogen for the Granzyme B antibody.

Storage

After reconstitution, the Granzyme B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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