GPRC5A Antibody Retinoic Acid Inducible GPCR Antibody FY12976

	Catalog No	Formulation	Size	Price (USD)
	FY12976	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml	100 ug	449
Formulation

GPRC5A Antibody Mouse Lung IHC. Immunohistochemistry analysis of FFPE mouse lung tissue using GPRC5A Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4Â°C. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen were used for visualization. Predominantly membranous staining is observed in alveolar and bronchiolar epithelial cells, consistent with the localization of GPRC5A as a retinoic acid-inducible orphan G protein-coupled receptor expressed in pulmonary epithelium. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for studying GPRC5A expression, epithelial GPCR biology and lung homeostasis.

GPRC5A Antibody Rat Lung IHC. Immunohistochemistry analysis of FFPE rat lung tissue using GPRC5A Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4Â°C. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen were used for visualization. Predominantly membranous staining is observed in alveolar and bronchiolar epithelial cells, consistent with the localization of GPRC5A as a retinoic acid-inducible orphan G protein-coupled receptor expressed in pulmonary epithelium. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for studying GPRC5A expression, epithelial GPCR biology and lung homeostasis.

GPRC5A Antibody Human Colon Cancer IHC. Immunohistochemistry analysis of FFPE human colon cancer tissue using GPRC5A Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4Â°C. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen were used for visualization. Predominantly membranous staining is observed in malignant epithelial cells, consistent with the localization of GPRC5A as a retinoic acid-inducible orphan G protein-coupled receptor. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for studying GPRC5A expression, epithelial GPCR biology and cancer-associated signaling.

GPRC5A Antibody Human Thyroid Cancer IHC. Immunohistochemistry analysis of FFPE human thyroid cancer tissue using GPRC5A Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4Â°C. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen were used for visualization. Predominantly membranous staining is observed in malignant epithelial cells, consistent with the localization of GPRC5A as a retinoic acid-inducible orphan G protein-coupled receptor expressed in epithelial tissues. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for studying GPRC5A expression, epithelial GPCR biology and cancer-associated signaling.

GPRC5A Antibody Human, Mouse and Rat WB. Western blot analysis using GPRC5A Antibody. Lane 1: human A375 cells; Lane 2: human MCF-7 cells; Lane 3: human HeLa cells; Lane 4: rat lung tissue; Lane 5: mouse lung tissue. Multiple immunoreactive bands are detected between approximately 37-50 kDa, consistent with the expected molecular weight of GPRC5A (~40 kDa) and the presence of different glycosylated or processed forms of this membrane-associated G protein-coupled receptor. Detection in human cell lines together with rat and mouse lung tissue supports cross-species reactivity. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for studying GPRC5A expression, epithelial GPCR biology and retinoic acid-regulated signaling.

GPRC5A Antibody HeLa Cell IP. Immunoprecipitation of GPRC5A from HeLa whole cell lysate using GPRC5A Antibody, followed by Western blot analysis with the same antibody. Lane 1: HeLa input lysate (30 ug); Lane 2: rabbit control IgG immunoprecipitation; Lane 3: GPRC5A immunoprecipitation using 2 ug of GPRC5A Antibody from 500 ug of HeLa lysate. A specific band is enriched between approximately 37-50 kDa in the GPRC5A immunoprecipitation sample, while no corresponding band is detected in the control IgG lane, confirming specific immunoprecipitation of GPRC5A. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for immunoprecipitation, protein interaction studies and GPCR signaling research.

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GPRC5A Antibody Mouse Lung IHC. Immunohistochemistry analysis of FFPE mouse lung tissue using GPRC5A Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen were used for visualization. Predominantly membranous staining is observed in alveolar and bronchiolar epithelial cells, consistent with the localization of GPRC5A as a retinoic acid-inducible orphan G protein-coupled receptor expressed in pulmonary epithelium. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for studying GPRC5A expression, epithelial GPCR biology and lung homeostasis.

GPRC5A Antibody Rat Lung IHC. Immunohistochemistry analysis of FFPE rat lung tissue using GPRC5A Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen were used for visualization. Predominantly membranous staining is observed in alveolar and bronchiolar epithelial cells, consistent with the localization of GPRC5A as a retinoic acid-inducible orphan G protein-coupled receptor expressed in pulmonary epithelium. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for studying GPRC5A expression, epithelial GPCR biology and lung homeostasis.

GPRC5A Antibody Human Colon Cancer IHC. Immunohistochemistry analysis of FFPE human colon cancer tissue using GPRC5A Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen were used for visualization. Predominantly membranous staining is observed in malignant epithelial cells, consistent with the localization of GPRC5A as a retinoic acid-inducible orphan G protein-coupled receptor. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for studying GPRC5A expression, epithelial GPCR biology and cancer-associated signaling.

GPRC5A Antibody Human Thyroid Cancer IHC. Immunohistochemistry analysis of FFPE human thyroid cancer tissue using GPRC5A Antibody. Heat induced antigen retrieval was performed in pH 8.0 EDTA buffer prior to overnight incubation with primary antibody (2 ug/mL) at 4°C. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen were used for visualization. Predominantly membranous staining is observed in malignant epithelial cells, consistent with the localization of GPRC5A as a retinoic acid-inducible orphan G protein-coupled receptor expressed in epithelial tissues. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for studying GPRC5A expression, epithelial GPCR biology and cancer-associated signaling.

GPRC5A Antibody Human, Mouse and Rat WB. Western blot analysis using GPRC5A Antibody. Lane 1: human A375 cells; Lane 2: human MCF-7 cells; Lane 3: human HeLa cells; Lane 4: rat lung tissue; Lane 5: mouse lung tissue. Multiple immunoreactive bands are detected between approximately 37-50 kDa, consistent with the expected molecular weight of GPRC5A (~40 kDa) and the presence of different glycosylated or processed forms of this membrane-associated G protein-coupled receptor. Detection in human cell lines together with rat and mouse lung tissue supports cross-species reactivity. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for studying GPRC5A expression, epithelial GPCR biology and retinoic acid-regulated signaling.

GPRC5A Antibody HeLa Cell IP. Immunoprecipitation of GPRC5A from HeLa whole cell lysate using GPRC5A Antibody, followed by Western blot analysis with the same antibody. Lane 1: HeLa input lysate (30 ug); Lane 2: rabbit control IgG immunoprecipitation; Lane 3: GPRC5A immunoprecipitation using 2 ug of GPRC5A Antibody from 500 ug of HeLa lysate. A specific band is enriched between approximately 37-50 kDa in the GPRC5A immunoprecipitation sample, while no corresponding band is detected in the control IgG lane, confirming specific immunoprecipitation of GPRC5A. These results support the use of GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody for immunoprecipitation, protein interaction studies and GPCR signaling research.

Availability	1-2 days
Species Reactivity	Human, Mouse, Rat
Format	Lyophilized
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Immunogen affinity purified
Buffer	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
UniProt	Q8NFJ5
Applications	Western Blot : 0.25-0.5ug/ml Immunohistochemistry : 2-5ug/ml Immunprecipitation : 2ug per 500ug of lysate
Limitations	This GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody is available for research use only.
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Description

GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody detects G protein-coupled receptor class C group 5 member A, an orphan receptor encoded by the GPRC5A gene. GPRC5A is also known as retinoic acid induced gene 1, RAIG1 and RAI3. It is a membrane-associated protein whose expression is strongly influenced by retinoic acid and is particularly associated with epithelial tissues. GPRC5A contains seven predicted transmembrane regions characteristic of G protein-coupled receptors and participates in pathways related to epithelial differentiation, cellular communication and tissue homeostasis. Its expression is especially prominent in pulmonary epithelium, making GPRC5A an important target in lung biology and epithelial cell research.

GPRC5A is classified as an orphan member of the class C GPCR family because a definitive endogenous ligand has not been established. Unlike many conventional GPCRs, GPRC5A has a relatively short extracellular amino-terminal region and may regulate cellular behavior through interactions with membrane proteins and intracellular signaling networks. Retinoic acid can induce GPRC5A transcription during epithelial differentiation, connecting vitamin A signaling with changes in receptor expression and tissue-specific cellular programs. A GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody is useful for investigating receptor localization, epithelial signaling and mechanisms through which retinoic acid influences cellular differentiation and function.

GPRC5A has received considerable attention in cancer research because altered expression has been reported in several epithelial malignancies. Experimental studies have supported a tumor suppressor role for GPRC5A in lung tissue, where loss or reduced expression may contribute to abnormal epithelial proliferation, inflammatory signaling and tumor development. Changes in GPRC5A expression have also been examined in breast, colon, pancreatic, gastric and head and neck cancers. Its effects may vary according to tissue type, cellular environment and disease stage, making antibody-based analysis valuable for comparing expression patterns across normal and malignant specimens.

Research into GPRC5A function has connected this receptor with signaling pathways involved in growth control, stress responses, inflammation and epithelial maintenance. GPRC5A may influence communication between membrane receptors and downstream pathways that regulate proliferation, apoptosis and cellular adaptation. Because respiratory epithelium is continuously exposed to environmental stressors, regulated GPRC5A expression may help maintain pulmonary tissue integrity and normal epithelial behavior. GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody supports studies examining lung homeostasis, epithelial transformation and the molecular consequences of altered orphan GPCR expression.

NSJ Bioreagents offers GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody products for researchers studying retinoic acid signaling, epithelial biology, GPCR function and cancer-associated pathways. These antibodies support applications including Western blot, immunohistochemistry and immunoprecipitation analysis of GPRC5A expression and localization in human, rat and mouse samples. GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody provides a valuable tool for investigating pulmonary epithelial biology, receptor regulation, tumor suppressor pathways and disease-associated changes in cellular signaling.

Researchers studying epithelial biology and GPCR signaling may also be interested in our Cancer Antibodies page, featuring antibodies against tumor suppressors, signaling proteins and other biomarkers involved in epithelial transformation, tumor progression and therapeutic research.

Application Notes

Optimal dilution of the GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody should be determined by the researcher.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human RAI3/GPRC5A was used as the immunogen for the GPRC5A antibody.

Storage

After reconstitution, the GPRC5A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

RAIG1 antibody, Retinoic Acid Induced Gene 1 antibody, Retinoic Acid Inducible Gene 1 antibody, G Protein Coupled Receptor Family C Group 5 Member A antibody, GPCR Family C Group 5 Member A antibody, RAI3 antibody

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GPRC5A Antibody / Retinoic Acid Inducible GPCR Antibody (FY12976)

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