- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
gp100 Melanoma Marker Antibody / PMEL [clone MSVA-617R] (V6111)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V6111-100UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 100 ug | 599.50 | |
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V6111-20UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 20 ug | 324.50 |
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| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | MSVA-617R |
| UniProt | P40967 |
| Localization | Endoplasmic reticulum membrane, Endosome, Golgi apparatus, Melanosome, Multivesicular body, Secreted |
| Applications | Immunohistochemistry (FFPE) : 1:100-1:200 |
| Limitations | This gp100 Melanoma Marker/PMEL antibody is available for research use only. |
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gp100 Melanoma Marker antibody recognizes Premelanosome protein, encoded by the PMEL gene and widely known as gp100, Pmel17, SILV, and Silver locus protein homolog. PMEL is a melanocyte lineage-specific type I transmembrane glycoprotein that localizes to early stage melanosomes within melanocytes and melanoma cells. gp100 Melanoma Marker antibody detects this structurally important melanosomal protein that plays a central role in pigment organelle maturation and melanocytic differentiation.
PMEL is synthesized in the endoplasmic reticulum and transported through the Golgi apparatus before being delivered to developing melanosomes. Within stage I and II melanosomes, the protein undergoes regulated proteolytic processing into fragments that assemble into amyloid-like fibrils. These fibrillar structures form the internal matrix of the melanosome and serve as a scaffold for eumelanin deposition. Proper PMEL processing is essential for normal pigmentation and helps compartmentalize reactive melanin intermediates during pigment synthesis.
Functionally, PMEL expression is largely restricted to melanocytes in the skin, hair follicles, and uveal tract, as well as to melanocytic tumors. Because of this lineage specificity, gp100 Melanoma Marker antibody is widely used in research settings to identify melanocytic cells and to characterize melanoma. Strong cytoplasmic and membranous staining is typically observed in melanoma tissue, while most non-melanocytic tissues demonstrate minimal or no expression, supporting its role as a melanocytic marker.
The PMEL gene is located on chromosome 12 and is a key component of the melanosome biogenesis pathway. Alterations in PMEL expression or processing can affect melanosome structure and pigmentation phenotypes. In melanoma biology, gp100 serves as a differentiation antigen and is frequently evaluated in studies examining tumor origin, lineage commitment, and melanocytic tumor characterization.
The gp100 Melanoma Marker antibody (Clone MSVA-617R) is a rabbit monoclonal antibody suitable for detecting PMEL protein expression in research applications. This PMEL antibody supports studies of melanocyte biology, melanosome structure, pigmentation pathways, and melanoma-related investigations.
1. Optimal dilution of the gp100 Melanoma Marker/PMEL antibody should be determined by the researcher.
2. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
A recombinant fragment (around amino acids 376-502) of human SILV protein (exact sequence is proprietary) was used as the immunogen for the gp100 Melanoma Marker/PMEL antibody.
gp100 Melanoma Marker/PMEL antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.
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