GOLPH3 Antibody / Golgi Phosphoprotein 3 Antibody (RQ4571)

GOLPH3 Antibody in Human Cancer Cell Lines and Placenta WB. Western blot testing of GOLPH3 Antibody / Golgi Phosphoprotein 3 Antibody at 0.5 ug/ml using human lysates from 1) HeLa cells, 2) placenta, 3) U-2 OS cells, 4) Caco-2 cells, 5) SW620 cells and 6) A549 cells. A prominent band is detected at approximately 34 kDa, consistent with the predicted molecular weight of GOLPH3. This GOLPH3 Antibody demonstrates detection of Golgi Phosphoprotein 3 across multiple human cancer cell lines and placenta tissue.

GOLPH3 Antibody in Rat and Mouse Tissues WB. Western blot testing of GOLPH3 Antibody / Golgi Phosphoprotein 3 Antibody at 0.5 ug/ml using lysates from 1) rat heart, 2) rat brain, 3) rat stomach, 4) rat testis, 5) mouse heart, 6) mouse brain, 7) mouse stomach, 8) mouse testis and 9) mouse NIH3T3 cells. A prominent band is detected at approximately 34 kDa, consistent with the predicted molecular weight of GOLPH3. This GOLPH3 Antibody demonstrates detection of Golgi Phosphoprotein 3 across multiple rat and mouse tissues and cells.

GOLPH3 Antibody in Human, Rat and Mouse Samples WB. Western blot testing of GOLPH3 Antibody / Golgi Phosphoprotein 3 Antibody at 0.5 ug/ml using lysates from 1) human HeLa cells, 2) human 293T cells, 3) human MDA-MB-453 breast cancer cells, 4) human A431 epidermoid carcinoma cells, 5) rat lung, 6) rat testis, 7) mouse lung and 8) mouse testis. A prominent band is detected at approximately 34 kDa, consistent with the predicted molecular weight of GOLPH3. This GOLPH3 Antibody demonstrates detection of Golgi Phosphoprotein 3 in human, rat and mouse samples.

GOLPH3 Antibody in Human A431 Epidermoid Carcinoma Cells IF. Immunofluorescence staining of FFPE human A431 epidermoid carcinoma cells with GOLPH3 Antibody / Golgi Phosphoprotein 3 Antibody (green). Heat-induced epitope retrieval (HIER) was performed by steaming in pH 6 citrate buffer for 20 min. Nuclei were counterstained with DAPI (blue). GOLPH3 staining is concentrated in discrete perinuclear structures, consistent with its localization to the Golgi apparatus. This GOLPH3 Antibody enables visualization of Golgi Phosphoprotein 3 expression and localization in human epidermoid carcinoma cells.

GOLPH3 Antibody in Human Intestinal Cancer IHC. Immunohistochemistry staining of FFPE human intestinal cancer tissue with GOLPH3 Antibody / Golgi Phosphoprotein 3 Antibody. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA for 20 min, followed by cooling before testing. GOLPH3 staining is observed in the intestinal tumor cells. This GOLPH3 Antibody demonstrates detection of Golgi Phosphoprotein 3 in human intestinal cancer tissue.

GOLPH3 Antibody in Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain tissue with GOLPH3 Antibody / Golgi Phosphoprotein 3 Antibody. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA for 20 min, followed by cooling before testing. GOLPH3 staining is observed in cells throughout the brain tissue, with prominent staining in a subset of cells. This GOLPH3 Antibody demonstrates detection of Golgi Phosphoprotein 3 in mouse brain tissue.

GOLPH3 Antibody in Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue with GOLPH3 Antibody / Golgi Phosphoprotein 3 Antibody. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA for 20 min, followed by cooling before testing. GOLPH3 staining is observed in neuronal populations within the brain tissue. This GOLPH3 Antibody demonstrates detection of Golgi Phosphoprotein 3 in rat brain tissue.

GOLPH3 Antibody Immunoprecipitation from Human HeLa Cells IP. Immunoprecipitation of GOLPH3 from human HeLa whole cell lysate was performed using GOLPH3 Antibody / Golgi Phosphoprotein 3 Antibody. Lane 1 contains 30 ug of HeLa whole cell lysate, lane 2 is the rabbit IgG control and lane 3 contains GOLPH3 immunoprecipitated from 500 ug of HeLa lysate using 2 ug of antibody. Western blot detection was performed with the antibody at 0.5 ug/ml followed by goat anti-rabbit IgG-HRP and ECL detection. A specific GOLPH3 band is detected at approximately 36 kDa, close to its expected molecular weight of approximately 34 kDa. This GOLPH3 Antibody demonstrates immunoprecipitation of Golgi Phosphoprotein 3 from human HeLa cells.