GNAQ Antibody / Guanine nucleotide binding protein Gq subunit alpha [clone 13H4] (FY13427)

Immunohistochemical staining of FFPE human ovarian cancer tissue using GNAQ antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing. Guanine nucleotide binding protein Gq subunit alpha shows cytoplasmic staining in tumor epithelial cells, with comparatively lower signal in surrounding stromal regions.

Immunohistochemical staining of FFPE human ovarian cancer tissue using GNAQ antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing. Guanine nucleotide binding protein Gq subunit alpha is detected with diffuse cytoplasmic staining across malignant glandular structures, while adjacent non-neoplastic tissue shows reduced signal intensity.

Immunohistochemical staining of FFPE mouse testis tissue with GNAQ antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot analysis of GNAQ using anti-GNAQ antibody. Samples include human Jurkat, HeLa, A549, A431, MCF-7, K562, and monkey COS7 cell lysates, as well as rat brain tissue lysate, rat lung tissue lysate, mouse brain tissue lysate, and mouse lung tissue lysate. Guanine nucleotide binding protein Gq subunit alpha has a predicted molecular weight of approximately 42 kDa and is observed as a primary band at approximately 42 kDa across all samples. In some lysates, GNAQ appears as a closely spaced doublet, which can be attributed to differential post-translational modifications such as phosphorylation or variable lipid modification states that alter electrophoretic mobility without changing core protein size.

Flow cytometry analysis of fixed and permeabilized human U-2 OS cells with GNAQ antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GNAQ antibody.