GM-CSF Antibody / CSF2 Microarray Specificity Validated Antibody [clone CSF2/3403] (V8121)

GM-CSF Antibody Lymphoid Tissue IHC. Immunohistochemistry of Granulocyte macrophage colony stimulating factor / CSF2 in FFPE human spleen tissue using mouse monoclonal GM-CSF antibody, clone CSF2/3403. HRP-DAB brown staining highlights cytoplasmic labeling in immune cell populations within splenic white pulp regions, consistent with cytokine expression in lymphoid tissue, while surrounding areas show lower background staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling prior to staining.

GM-CSF Antibody Immune Cell Distribution IHC. Immunohistochemistry of Granulocyte macrophage colony stimulating factor / CSF2 in FFPE human spleen tissue using mouse monoclonal GM-CSF antibody, clone CSF2/3403. HRP-DAB brown staining highlights scattered cytoplasmic labeling in subsets of immune cells within splenic tissue, with a focal and heterogeneous distribution consistent with cytokine-producing cell populations, while surrounding lymphoid cells show lower staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling prior to staining.

GM-CSF Antibody Lymphoid Follicle IHC. Immunohistochemistry of Granulocyte macrophage colony stimulating factor / CSF2 in FFPE human spleen tissue using mouse monoclonal GM-CSF antibody, clone CSF2/3403. HRP-DAB brown staining highlights dense cytoplasmic labeling within lymphoid follicle regions, with strong signal in clustered immune cells consistent with localized cytokine expression, while surrounding splenic tissue shows lower background staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling prior to staining.

GM-CSF Antibody Microarray Specificity Validation. Protein microarray analysis of Granulocyte macrophage colony stimulating factor / CSF2 using mouse monoclonal GM-CSF antibody, clone CSF2/3403, across a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding to CSF2 with minimal off-target interaction. The signal profile shows strong enrichment for CSF2 relative to other proteins on the array, supporting high specificity. Z-score represents the strength of antibody binding signal expressed as standard deviations above the mean array signal, while S-score reflects the separation between the top-ranked target and the next highest signal, confirming selective recognition of GM-CSF in a proteome-wide context.

SDS-PAGE analysis of purified, BSA-free GMCSF antibody (clone CSF2/3403) as confirmation of integrity and purity.