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Home >> Antibodies >> Glypican 5 Antibody / GPC5

Glypican 5 Antibody / GPC5 (RQ5885)

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  Catalog No Formulation Size Price (USD)  
Image RQ5885 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human U-87 MG cells with Glypican 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glypican 5 antibody.
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Flow cytometry testing of human U-87 MG cells with Glypican 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glypican 5 antibody.
Flow cytometry testing of human U-2 OS cells with Glypican 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glypican 5 antibody.
Western blot testing of human 1) HeLa, 2) U-87 MG and 3) U-2 OS lysate with Glypican 5 antibody. Predicted molecular weight ~64 kDa.
Immunofluorescent staining of FFPE human A431 cells with Glypican 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P78333
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Glypican 5 antibody is available for research use only.
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Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Application Notes

Optimal dilution of the Glypican 5 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Q28-D538) was used as the immunogen for the Glypican 5 antibody.

Storage

After reconstitution, the Glypican 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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