- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Glypican 3 Antibody [clone SPM595] (V2537CF555)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V2537CF555-100T | 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 Tests | 539 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | CF555 Conjugate |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | SPM595 |
| Purity | Protein G affinity chromatography |
| UniProt | P51654 |
| Localization | Cytoplasmic |
| Applications | Flow Cytometry : 5ul/test/million cells in 0.1ml Immunofluorescence : 1-2ug/ml |
| Limitations | This Glypican 3 antibody is available for research use only. |
|
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The Glypican 3 (GPC3) protein is a heparan sulfate proteoglycan anchored to the cell membrane through a glycosylphosphatidylinositol (GPI) linkage. It participates in the regulation of major signaling pathways such as Wnt, Hedgehog, and fibroblast growth factor, which control cell growth and differentiation. A CF555 Glypican 3 antibody provides the added advantage of direct fluorescence labeling, making it well suited for imaging applications.
GPC3 expression is prominent during embryonic development, where it helps guide tissue morphogenesis and organ growth. In healthy adults, levels of Glypican 3 are usually very low, but re-expression occurs in several tumor types, most notably hepatocellular carcinoma, hepatoblastoma, and certain testicular cancers. This makes a CF555 Glypican 3 antibody an important reagent for investigating tumor biology, biomarker discovery, and cancer diagnostics.
The CF555 dye produces a bright orange-red fluorescence signal, allowing for sensitive detection in immunofluorescence microscopy, flow cytometry, and other fluorescence-based techniques. Using a CF555 Glypican 3 antibody eliminates the need for secondary antibody staining and helps reduce background signal while improving workflow efficiency.
NSJ Bioreagents offers a CF555-conjugated Glypican 3 antibody designed to deliver consistent, reproducible results. Researchers can apply this antibody to studies in cancer research, developmental biology, and signaling pathway analysis where direct fluorescent detection is beneficial.
Optimal dilution of the Glypican 3 antibody should be determined by the researcher.
A recombinant fragment containing amino acids 511-580 from the human protein was used as the immunogen for the Glypican-3 antibody.
Store the Glypican 3 antibody at 2-8oC, protected from light.
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