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Home >> Antibodies >> Glyoxalase I Antibody / GLO1

Glyoxalase I Antibody / GLO1 (F54301)

  Catalog No Formulation Size Price (USD)  
Image F54301-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54301-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with Glyoxalase I antibody. Predicted molecular weight ~21 kDa.
Western blot testing of human HL60 cell lysate with Glyoxalase I antibody. Predicted molecular weight ~21 kDa.
IHC testing of FFPE human brain tissue with Glyoxalase I antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human WiDr cells with Glyoxalase I antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of fixed and permeabilized human WiDr cells with Glyoxalase I antibody; Blue=isotype control, Green= Glyoxalase I antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity SAS precipitation
UniProt Q04760
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Immunofluorescence : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Limitations This Glyoxalase I antibody is available for research use only.
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Description

The enzyme GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Glyoxalase I antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 23-51 from the human protein were used as the immunogen for the Glyoxalase I antibody.

Storage

Aliquot the Glyoxalase I antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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