Glutamic-oxaloacetic transaminase 1 Antibody / GOT1 (RQ6423)

Description

Glutamic-oxaloacetic transaminase 1 (GOT1), also known as cytosolic aspartate aminotransferase, is a pyridoxal phosphate-dependent enzyme that catalyzes the reversible transamination between aspartate and alpha-ketoglutarate to generate oxaloacetate and glutamate. GOT1 plays a central role in amino acid metabolism, the malate-aspartate shuttle, and the coordination of cellular redox balance.

GOT1 is broadly expressed across tissues and contributes to linking nitrogen metabolism with energy production. Its activity supports mitochondrial function indirectly through shuttling reducing equivalents, making it an important enzyme in bioenergetics and metabolic regulation. GOT1 levels and activity are frequently measured in biochemical studies focused on cellular metabolism and enzymatic pathway integration.

The Glutamic-oxaloacetic transaminase 1 antibody is a reliable reagent for detecting endogenous GOT1 in applications such as western blot, immunohistochemistry, and immunofluorescence. Researchers use the Glutamic-oxaloacetic transaminase 1 antibody from NSJ Bioreagents to quantify protein abundance, determine subcellular localization, and assess metabolic changes under defined experimental conditions. With high specificity and consistent performance, the Glutamic-oxaloacetic transaminase 1 antibody supports rigorous studies in enzymology, metabolic flux analysis, and amino acid pathway research.

Application Notes

Optimal dilution of the Glutamic-oxaloacetic transaminase 1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids S5-Q413) was used as the immunogen for the Glutamic-oxaloacetic transaminase 1 antibody.

Storage

After reconstitution, the Glutamic-oxaloacetic transaminase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.