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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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Glucose transporter 1 (SLC2A1), widely known as GLUT1, is a facilitative glucose transporter responsible for basal glucose uptake in many tissues and plays a key role in cellular energy metabolism. GLUT1 Antibody for IF (clone CGG-19) enables immunofluorescence-based visualization of this membrane-associated glucose transporter and supports fluorescence microscopy analysis of GLUT1 localization in cultured cells and tissue sections. In immunofluorescence staining experiments, GLUT1 typically appears as strong fluorescent labeling along the plasma membrane outlining individual cells, reflecting localization of the transporter at the cell surface where glucose uptake occurs. Because GLUT1 functions primarily at the plasma membrane, immunofluorescence microscopy commonly reveals clear membrane-associated fluorescence that delineates cellular boundaries and highlights glucose transporter distribution within metabolically active cells.
Immunofluorescence analysis of SLC2A1 is widely used to study glucose transport regulation, membrane transporter trafficking, and metabolic signaling pathways. Using fluorescence microscopy, GLUT1 staining often produces a characteristic combination of membrane-associated fluorescence together with punctate cytoplasmic fluorescent structures representing intracellular transporter pools. These vesicular fluorescent signals correspond to transporter trafficking pathways in which GLUT1 cycles between intracellular vesicles and the plasma membrane. A GLUT1 Antibody for IF therefore allows researchers to visualize both membrane-localized glucose transporters and intracellular vesicular compartments involved in transporter recycling.
Confocal immunofluorescence microscopy enables high-resolution analysis of GLUT1 subcellular localization and is frequently used to examine plasma membrane transporter organization in cultured cells. In confocal imaging experiments, GLUT1 immunofluorescence often appears as continuous fluorescent signal outlining the cell membrane combined with vesicular fluorescent puncta distributed throughout the cytoplasm. These imaging patterns reflect dynamic transporter trafficking and membrane insertion events that regulate glucose uptake under different metabolic conditions. Fluorescence microscopy therefore provides a powerful approach for studying the spatial organization of glucose transport systems within cells.
Immunofluorescence detection of GLUT1 can also be incorporated into co-localization studies with plasma membrane markers, endosomal proteins, or metabolic signaling regulators to investigate transporter trafficking and membrane dynamics. Because GLUT1 expression is frequently elevated in rapidly proliferating cells and many tumor cell types, immunofluorescence staining of SLC2A1 is widely used to visualize metabolic adaptation and glucose transporter redistribution during oncogenic signaling and hypoxia-associated responses. Fluorescent labeling of GLUT1 therefore provides insight into metabolic regulation and membrane transporter biology in both normal and cancer cells.
GLUT1 Antibody for IF (clone CGG-19) supports immunofluorescence-based detection of SLC2A1 using fluorescence microscopy and enables detailed visualization of plasma membrane localization, intracellular transporter trafficking, and metabolic transporter distribution. Immunofluorescence imaging using a GLUT1 antibody typically reveals membrane-associated fluorescent signal outlining individual cells together with vesicular cytoplasmic fluorescence, providing a powerful tool for studying glucose transporter localization, cellular metabolism, and glucose uptake pathways.
Optimal dilution of the GLUT1 Antibody for IF should be determined by the researcher.
A synthetic peptide specific to human GLUT1 / SLC2A1 was used as the immunogen for the GLUT1 antibody.
Store the GLUT1 antibody at -20oC.
SLC2A1 antibody, Glucose transporter 1 antibody, GLUT-1 antibody, Erythrocyte glucose transporter antibody
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