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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
GLUL Antibody Rabbit Polyclonal / Glutamine Synthetase (RQ6636)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ6636 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | P15104 |
| Localization | Cytoplasmic |
| Applications | Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence (FFPE) : 5ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml |
| Limitations | This GLUL antibody is available for research use only. |
|
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Glutamate-ammonia ligase is a cytosolic enzyme encoded by the GLUL gene and widely known as Glutamine synthetase. GLUL Antibody Rabbit Polyclonal is developed to detect this key metabolic enzyme, which catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine. This reaction is essential for nitrogen metabolism, ammonia detoxification, and regulation of intracellular glutamine pools. The GLUL gene is located on chromosome 1q31.3 and demonstrates regulated, tissue-specific expression patterns that reflect metabolic demand and cellular specialization.
Glutamine synthetase displays highly characteristic distribution across multiple tissues. In the liver, GLUL expression is largely restricted to pericentral hepatocytes surrounding central veins, forming a sharply defined zonal staining pattern within hepatic lobules. This pattern reflects metabolic compartmentalization in the liver and is frequently used in research evaluating hepatic architecture, metabolic regulation, and tumor-associated signaling pathways. In the central nervous system, the enzyme is highly enriched in astrocytes where it participates in the glutamate-glutamine cycle and supports neurotransmitter recycling. In these glial cells, GLUL localizes to the cytoplasm and extends throughout the astrocytic processes within the neuropil.
Expression can also be observed in kidney, skeletal muscle, and selected epithelial tissues depending on physiological context. In cancer research, altered glutamine synthetase levels have been reported in hepatocellular carcinoma and other malignancies where GLUL upregulation may be associated with metabolic reprogramming and pathway activation. These biologically distinct expression patterns make GLUL a valuable marker for studies examining metabolic zonation, astrocyte biology, and tumor-associated metabolic adaptation.
As a rabbit polyclonal reagent, GLUL Antibody Rabbit Polyclonal recognizes glutamine synthetase and supports investigation of cytoplasmic GLUL expression in normal tissues and disease-associated contexts. Positive cells typically demonstrate diffuse to granular cytoplasmic staining consistent with the known intracellular distribution of glutamine synthetase, enabling analysis of metabolic regulation and tissue-specific expression patterns.
Optimal dilution of the GLUL antibody rabbit polyclonal should be determined by the researcher.
Recombinant human protein (amino acids N74-N373) was used as the immunogen for the GLUL antibody.
After reconstitution, the GLUL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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