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Home >> Antibodies >> Glutamine Synthetase Antibody / GLUL

Glutamine Synthetase Antibody / GLUL [clone GLUL/6599] (V9622)

  Catalog No Formulation Size Price (USD)  
Image V9622-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9622-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9622SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human liver tissue using GLUL antibody (clone GLUL/6599). Predicted molecular weight ~42 kDa.
IHC staining of FFPE human liver tissue with GLUL antibody (clone GLUL/6599) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free GLUL antibody (clone GLUL/6599) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name GLUL/6599
Purity Protein A/G affinity
UniProt P15104
Localization Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This GLUL antibody is available for research use only.
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Description

Glutamine synthetase (GlSyn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.

Application Notes

Optimal dilution of the GLUL antibody should be determined by the researcher.

Immunogen

A portion of amino acids 50-250 was used as the immunogen for the GLUL antibody.

Storage

Aliquot the GLUL antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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