GPI Antibody / Glucose 6-Phosphate Isomerase Antibody [clone CPTC-GPI-1] (V7817)

GPI Antibody Human Breast Carcinoma IHC. Immunohistochemistry was performed on FFPE human breast carcinoma tissue using GPI Antibody (clone CPTC-GPI-1). Heat-induced epitope retrieval was carried out in pH 9 Tris-EDTA buffer prior to antibody incubation. Bound antibody was visualized using an HRP-based detection system with DAB chromogen. Strong cytoplasmic staining is observed throughout the neoplastic epithelial cells, consistent with the elevated expression of Glucose 6-Phosphate Isomerase frequently associated with increased glycolytic activity in malignant tissues. These results demonstrate that this Glucose 6-Phosphate Isomerase Antibody, Protein Microarray Validated with clone CPTC-GPI-1, is suitable for immunohistochemical detection of GPI in formalin-fixed, paraffin-embedded human breast carcinoma tissue.

GPI Antibody MCF-7 Cell IF. Immunofluorescence was performed on fixed human MCF-7 cells using GPI Antibody (clone CPTC-GPI-1, green). Nuclei were counterstained with RedDot (red). Strong diffuse cytoplasmic staining is observed throughout the cells with minimal nuclear signal, consistent with the predominant cytosolic localization of Glucose 6-Phosphate Isomerase as a key glycolytic enzyme. These results demonstrate that this Glucose 6-Phosphate Isomerase Antibody, Protein Microarray Validated with clone CPTC-GPI-1, is suitable for immunofluorescent detection of endogenous GPI in human MCF-7 cells.

GPI Antibody Human Cancer Cell WB. Western blot analysis was performed using GPI Antibody. Lane 1: human T-47D cell lysate; lane 2: human PC-3 cell lysate; lane 3: human HepG2 cell lysate. A specific immunoreactive band is detected at approximately 63 kDa in all three human cancer cell lines, consistent with the expected molecular weight of Glucose 6-Phosphate Isomerase. These results demonstrate that this Glucose 6-Phosphate Isomerase Antibody, Protein Microarray Validated, is suitable for Western blot detection of endogenous GPI in human cancer cell lysates.

GPI Antibody Protein Microarray Validation. Specificity of GPI Antibody (clone CPTC-GPI-1) was evaluated using the HuProt human protein microarray containing more than 19,000 full-length human proteins. The antibody demonstrated exceptional target specificity, with GPI ranked as the highest-scoring protein (Z-score: 82.35; S-score: 77.39), while all non-target proteins produced substantially lower signals. The Z-score reflects the strength of antibody binding relative to the array background, whereas the S-score measures target specificity by comparing the intended target with the next highest-ranking protein. These results demonstrate that this Glucose 6-Phosphate Isomerase Antibody, Protein Microarray Validated with clone CPTC-GPI-1, exhibits outstanding specificity for GPI with minimal off-target reactivity.

SDS-PAGE analysis of purified, BSA-free Glucose 6-Phosphate Isomerase antibody (clone CPTC-GPI-1) as confirmation of integrity and purity.

GPI Antibody Human Kidney WB. Western blot analysis was performed on human kidney tissue lysate using GPI Antibody. A specific immunoreactive band is detected at approximately 63 kDa, consistent with the expected molecular weight of Glucose 6-Phosphate Isomerase. The blot demonstrates robust detection of endogenous GPI with minimal background, supporting reliable recognition of this abundantly expressed glycolytic enzyme in human tissue. These results demonstrate that this Glucose 6-Phosphate Isomerase Antibody, Protein Microarray Validated, is suitable for Western blot detection of endogenous GPI in human kidney tissue lysates.