GLS Antibody / Glutaminase (RQ5691)

Immunofluorescent staining of FFPE human U-2 OS cells with GLS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human stomach cancer with GLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat brain with GLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human placenta, 2) human U-87 MG, 3) human HeLa, 4) monkey kidney, 5) rat brain, 6) rat kidney, 7) rat heart, 8) rat skeletal muscle, 9) mouse brain, 10) mouse kidney, 11) mouse heart, 12) mouse skeletal muscle and 13) mouse Neuro-2a antibody. Predicted molecular weigh: 65-73 kDa.

Flow cytometry testing of human 293T cells with GLS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GLS antibody.

Flow cytometry testing of human SiHa cells with GLS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GLS antibody.