- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
GLI1 Antibody / Glioma-associated oncogene homolog 1 (FY12192)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12192 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
|
| Availability | 1-2 days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Lyophilized |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Immunogen affinity purified |
| Buffer | Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
| UniProt | P08151 |
| Applications | Western Blot : 0.25-0.5ug/ml ELISA : 0.1-0.5ug/ml |
| Limitations | This GLI1 antibody is available for research use only. |
|
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GLI1 antibody detects Glioma-associated oncogene homolog 1, encoded by the GLI1 gene on chromosome 12q13.3. GLI1 antibody is used in research on developmental signaling, transcriptional regulation, and cancer biology. GLI1 is a zinc finger transcription factor and a key effector of the Hedgehog signaling pathway, which controls cell fate, proliferation, and differentiation during embryogenesis. Aberrant activation of GLI1 contributes to tumorigenesis, particularly in basal cell carcinoma, medulloblastoma, and other cancers. GLI1 expression is found in embryonic tissues, stem cells, and tumor cells, with tightly regulated activity in development and disease.
Structurally, GLI1 contains five C2H2-type zinc finger DNA-binding motifs that recognize consensus sequences in Hedgehog target genes. Its N-terminal region regulates transcriptional activity, while the C-terminal domain contains transactivation motifs. GLI1 functions as a transcriptional activator, unlike GLI2 and GLI3 which can act as both activators and repressors. Regulation of GLI1 involves nuclear-cytoplasmic shuttling, post-translational modifications, and interaction with cofactors.
Functionally, GLI1 activates transcription of genes controlling proliferation, survival, and differentiation. In development, it directs patterning of the nervous system, limbs, and other organs. In adult tissues, GLI1 is normally quiescent but can be reactivated in stem cell niches. Dysregulation results in uncontrolled proliferation and cancer initiation. Researchers use GLI1 antibody to study Hedgehog signaling, transcriptional control, and oncogenesis.
Clinically, GLI1 is strongly linked to cancer. Overexpression or constitutive activation drives tumor growth in basal cell carcinoma, medulloblastoma, gliomas, and pancreatic cancer. GLI1 serves as a biomarker of Hedgehog pathway activity and a therapeutic target in oncology. Inhibitors targeting upstream components such as Smoothened indirectly reduce GLI1 activity, while direct GLI inhibitors are in development. GLI1 is also associated with developmental disorders caused by Hedgehog pathway disruption. NSJ Bioreagents provides GLI1 antibody for reliable detection in developmental biology and oncology research.
Experimentally, GLI1 antibody is used in western blotting to detect the ~110 kDa protein, in immunohistochemistry to analyze tumor expression, and in immunofluorescence microscopy to study nuclear localization. Immunoprecipitation with GLI1 antibody enables analysis of transcriptional complexes and cofactors. These applications provide a versatile toolkit for dissecting Hedgehog pathway regulation.
Optimal dilution of the GLI1 antibody should be determined by the researcher.
E.coli-derived human GLI1 recombinant protein (Position: Y800-Q1057) was used as the immunogen for the GLI1 antibody.
After reconstitution, the GLI1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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