- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
GKN1 Antibody / Gastrokine 1 (FY12711)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12711 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
GKN1 antibody detects Gastrokine-1 (also known as Antral mucosal protein or AMP-18), a secreted epithelial protein that promotes gastric mucosal integrity and repair. Encoded by the GKN1 gene on chromosome 2p13.3, this small peptide is abundantly expressed in the stomach epithelium, where it supports epithelial cell survival, migration, and restitution following injury. Gastrokine-1 contains a BRICHOS domain that mediates protein-protein interactions involved in epithelial protection and regeneration. It is secreted into the gastric lumen and acts both locally and paracrinally to maintain mucosal homeostasis.
GKN1 expression is restricted to normal gastric mucosa and is markedly downregulated or lost in gastric cancer, making it a useful biomarker for gastric differentiation and neoplastic transformation. Functional studies demonstrate that GKN1 suppresses gastric tumor cell proliferation, modulates NF-kB signaling, and inhibits epithelial-mesenchymal transition. Loss of GKN1 leads to increased susceptibility to inflammation-induced damage and carcinogenesis. By promoting mucosal healing and preserving epithelial barrier integrity, GKN1 functions as a tumor suppressor and protective factor in the gastrointestinal tract.
The GKN1 antibody is widely used in gastrointestinal biology, cancer, and inflammation research to detect Gastrokine-1 in tissue sections or cell lysates. In immunohistochemistry, GKN1 localizes predominantly to the cytoplasm and secretory granules of surface mucous cells. Western blotting identifies a 19 kilodalton band corresponding to the mature secreted form. The antibody assists in distinguishing normal gastric tissue from malignant lesions, as GKN1 expression is largely absent in gastric adenocarcinoma but retained in non-neoplastic mucosa. This differential expression pattern has diagnostic and prognostic implications.
Beyond the stomach, low levels of GKN1 are occasionally detected in duodenal and pancreatic tissues, suggesting secondary protective roles in the upper gastrointestinal tract. The protein also exhibits antibacterial activity and modulates host responses to Helicobacter pylori infection. Because of these diverse functions, the GKN1 antibody supports research into epithelial regeneration, cancer suppression, and gastric disease pathogenesis. NSJ Bioreagents provides this antibody validated for its applications, enabling accurate analysis of Gastrokine-1 expression in health and disease.
Optimal dilution of the GKN1 antibody should be determined by the researcher.
E.coli-derived human GKN1 recombinant protein (Position: M1-N185) was used as the immunogen for the GKN1 antibody.
After reconstitution, the GKN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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