GKAP1 Antibody / G kinase-anchoring protein 1 Antibody (FY12580)

GKAP1 Antibody Rat Testis IHC. Immunohistochemical staining of FFPE rat testis tissue using GKAP1 Antibody at 2 ug/ml with HRP/DAB detection following heat-induced epitope retrieval in pH8 EDTA buffer. Prominent staining is observed in seminiferous tubules, with strongest expression in developing germ cells, consistent with the reported role of GKAP1 in testicular function and intracellular signaling. This staining pattern demonstrates the utility of GKAP1 Antibody, a G Kinase-Anchoring Protein 1 Antibody, for investigating reproductive biology, germ cell development and protein kinase A signaling in tissue sections.

Western blot analysis of GKAP1 using GKAP1 Antibody / G kinase-anchoring protein 1 Antibody. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GKAP1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot analysis of rat and mouse testis lysates probed with anti-GKAP1 shows a major band at the expected ~46 kDa full-length protein and a lower ~38–40 kDa band corresponding to a truncated GKAP1 isoform expressed in testis.

GKAP1 Antibody Human and Rodent Testis WB. Western blot analysis of GKAP1 Antibody using lysates from lane 1: human U2OS cells, lane 2: rat testis and lane 3: mouse testis. A specific band is detected at approximately 42 kDa, consistent with the predicted molecular weight of endogenous GKAP1. An additional lower-molecular-weight band is observed in rat testis, which may represent a tissue-specific isoform or post-translationally processed form of the protein. These results demonstrate cross-species detection of GKAP1 and support the use of GKAP1 Antibody, a G Kinase-Anchoring Protein 1 Antibody, for studies of protein kinase A signaling, testicular biology and intracellular scaffold proteins.