GK5 Antibody / Glycerol Kinase 5 Antibody (FY12424)

GK5 Antibody JK Cell FACS. Flow cytometry analysis of fixed and permeabilized JK cells stained with GK5 Antibody at 1 ug/10^6 cells, followed by a DyLight 488-conjugated secondary antibody. The blue histogram (GK5) exhibits a clear rightward shift relative to the rabbit IgG isotype control (green) and unstained cells (red), demonstrating specific intracellular detection of endogenous GK5. These results support the use of GK5 Antibody, also known as Glycerol Kinase 5 Antibody, for analyzing GK5 expression and glycerol kinase family protein distribution by flow cytometry.

GK5 Antibody Human Cancer Cell WB. Western blot analysis of GK5 Antibody using lysates from lane 1: human Jurkat cells, lane 2: human A431 cells, lane 3: human SiHa cells and lane 4: human RT4 cells. Specific bands are detected between approximately 55-65 kDa, consistent with the expected molecular weight of endogenous GK5 (~61 kDa). The closely spaced doublet observed in some samples is consistent with differential mitochondrial processing and/or post-translational modification of GK5. These results demonstrate reliable detection of GK5 Antibody, also known as Glycerol Kinase 5 Antibody, for studies of GK5 expression and glycerol kinase family biology in human cells.