- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Gilt Antibody / Ifi30 / Gamma Interferon Inducible Lysosomal Thiol Reductase (RQ5820)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ5820 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Mouse, Rat |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide |
| UniProt | Q9ESY9 |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry : 1-2ug/ml Flow Cytometry : 1-3ug/million cells Immunofluorescence : 5ug/ml |
| Limitations | This Gilt antibody is available for research use only. |
|
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Gilt antibody targets Gamma Interferon Inducible Lysosomal Thiol Reductase, a lysosomal enzyme encoded by the Ifi30 gene that plays a central role in antigen processing within the major histocompatibility complex class II pathway. Gilt is primarily localized to endolysosomal compartments of antigen-presenting cells, including dendritic cells, macrophages, and B cells, where it functions in a reducing environment. Expression of Gilt is strongly upregulated by interferon gamma, linking its activity to immune activation and inflammatory signaling.
Functionally, Gamma Interferon Inducible Lysosomal Thiol Reductase catalyzes the reduction of disulfide bonds in internalized protein antigens, facilitating their unfolding and subsequent proteolytic processing. A short functional summary is that Gilt enables efficient generation of peptide epitopes for loading onto MHC class II molecules, thereby supporting CD4 positive T cell activation. Through this activity, Gilt is a critical regulator of adaptive immune responses and antigen presentation efficiency.
At the molecular level, Gilt is synthesized as a precursor protein that undergoes proteolytic maturation within lysosomes to generate the active enzyme. The protein contains a conserved Cys-X-X-Cys active site motif that mediates thiol reductase activity under acidic conditions. Gilt antibody reagents are widely used to study lysosomal function, antigen processing mechanisms, and the regulation of immune responses in both resting and activated antigen-presenting cells.
From a biological and disease relevance perspective, altered Gilt expression has been linked to immune dysregulation, autoimmunity, infection, and cancer. Reduced or aberrant expression of Gamma Interferon Inducible Lysosomal Thiol Reductase can impair antigen presentation and modify T cell responses, while increased expression is often observed in inflammatory environments. Gilt has also been investigated in tumor immunology, where changes in antigen processing pathways can influence immune recognition of malignant cells.
Developmentally, Gilt expression increases as antigen-presenting cells mature and acquire full immunostimulatory capacity. Its interferon responsiveness positions Gilt at the intersection of innate and adaptive immunity. Gilt antibody tools support research applications aimed at characterizing antigen processing pathways, lysosomal redox biology, and immune cell function. Gilt antibodies from NSJ Bioreagents are supplied for research use to support studies in immunology, inflammation, and antigen presentation.
Optimal dilution of the Gilt antibody should be determined by the researcher.
Amino acids QLMHENAQLTDALHPPHEY from the mouse protein were used as the immunogen for the Gilt antibody.
After reconstitution, the Gilt antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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