- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
GFP Antibody / YFP [clone 168AT1211] (F52058)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
F52058-0.2ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.2 ml | 429 | |
|
|
F52058-0.05ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.05 ml | 199 |
| Availability | 1-3 business days |
| Species Reactivity | GFP / YFP |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1 |
| Clone Name | 168AT1211 |
| Purity | Purified |
| Applications | Western Blot : 1:4000 Immunofluorescence/Immunocytochemistry : 1:25 |
| Limitations | This GFP antibody is available for research use only. |
|
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GFP antibody is a widely used reagent for detecting expression of green fluorescent protein in diverse biological systems. Green fluorescent protein (GFP) is a naturally occurring protein originally isolated from the jellyfish Aequorea victoria. Its ability to fluoresce without requiring cofactors has made GFP one of the most important tools in molecular and cellular biology. By fusing GFP to proteins of interest, researchers can monitor localization, dynamics, and interactions in living cells.
GFP has been engineered into numerous variants with altered excitation and emission spectra, enabling multi-color imaging and advanced microscopy applications. These derivatives, such as EGFP and superfolder GFP, improve brightness, folding efficiency, and stability, making them ideal for experimental design. Despite advances in fluorescent proteins, the original GFP remains the foundation for many constructs and reporter systems.
The GFP antibody is particularly valuable for detecting GFP fusion proteins in fixed cells and tissues or in western blotting. Antibody based detection overcomes challenges of weak fluorescence, photobleaching, or interference with protein folding. This allows researchers to confirm GFP fusion expression even when fluorescence is not detectable. Immunohistochemistry and immunofluorescence with GFP antibody provide higher sensitivity and versatility compared to relying on intrinsic fluorescence alone.
At the molecular level, GFP consists of 238 amino acids and forms a beta barrel structure surrounding a chromophore formed by cyclization of internal residues. This self-contained chromophore is stable and resistant to environmental changes, contributing to GFPâs utility as a reporter protein. Variants of GFP have been optimized for mammalian expression, photostability, and reduced cytotoxicity, broadening its applications across disciplines.
The GFP antibody is applied across cell biology, developmental biology, neuroscience, and immunology. It is used to detect tagged proteins in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. For researchers studying protein localization, gene expression, or signal transduction, the GFP antibody provides a sensitive and specific detection tool. NSJ Bioreagents supplies validated antibodies that ensure accuracy and reproducibility in advanced molecular studies.
Titration of the GFP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Purified His-tagged protein was used to produced this monoclonal YFP / GFP antibody.
Aliquot the GFP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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