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Home >> Antibodies >> GFAP Antibody

GFAP Antibody [clone 3F2] (RQ6032)

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  Catalog No Formulation Size Price (USD)  
Image RQ6032 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE rat brain with GFAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat brain with GFAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioma with GFAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain and 2) mouse brain lysate with GFAP antibody. Predicted molecular weight ~50 kDa.
Immunofluorescent staining of FFPE rat brain tissue with GFAP antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 3F2
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P14136
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Immunofluorescence : 5ug/ml
Limitations This GFAP antibody is available for research use only.
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Description

Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, and ependymal cells. It is mapped to 17q21.31. GFAP is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell's cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells. This gene has been shown to play a role in mitosis by adjusting the filament network present in the cell. GFAP is necessary for many critical roles in the CNS. What's more, GFAP also plays a role in astrocyte-neuron interactions as well as cell-cell communication.

Application Notes

Optimal dilution of the GFAP antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Q93-M432) was used as the immunogen for the GFAP antibody.

Storage

After reconstitution, the GFAP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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