GFAP Antibody / Glial Fibrillary Acidic Protein [clone GFAP/9423] (V5714)

GFAP Antibody Multi-Tissue WB. Western blot analysis of human brain, heart, intestine, kidney, liver, lung, ovary, pancreas, prostate, skeletal muscle, skin, spleen, stomach, and testis tissue lysates using GFAP Antibody clone GFAP/9423. A distinct band is detected at approximately 50 kDa selectively in human brain lysate, consistent with the predicted molecular weight of Glial fibrillary acidic protein / GFAP, an astrocyte-associated intermediate filament protein enriched within central nervous system tissue. Multiple closely migrating lower molecular weight bands forming a stacked pattern beneath the primary signal likely represent GFAP isoforms and/or partial proteolytic processing products commonly observed in neural tissue extracts.

IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/9423). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GFAP/9423) as confirmation of integrity and purity.

Western blot testing of human brain tissue with GFAP antibody (clone GFAP/9423). Predicted molecular weight ~50 kDa.

GFAP Antibody Brain Tissue WB. Western blot analysis of human brain, mouse brain, rat brain, hamster brain, guinea pig brain, and rabbit brain tissue lysates using GFAP Antibody clone GFAP/9423. Multiple closely migrating bands are detected between approximately 40-50 kDa in human brain lysate, with additional bands of similar molecular weight observed in rat, guinea pig, and rabbit brain samples, consistent with Glial fibrillary acidic protein / GFAP, a major astrocytic intermediate filament protein involved in maintenance of neural cytoskeletal organization and central nervous system architecture. The stacked banding pattern in the human brain sample likely reflects a combination of GFAP isoforms and partial proteolytic processing products commonly observed in neural tissue extracts.