GFAP Antibody / Glial Fibrillary Acidic Protein [clone GFAP/9422] (V5713)

IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/9422). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human brain tissue with GFAP antibody (clone GFAP/9422). Predicted molecular weight ~50 kDa.

SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GFAP/9422) as confirmation of integrity and purity.

GFAP Antibody Brain Tissue WB. Western blot analysis of human brain, mouse brain, rat brain, hamster brain, guinea pig brain, and rabbit brain tissue lysates using GFAP Antibody clone GFAP/9422. Multiple closely migrating bands are detected between approximately 40-50 kDa in human brain lysate, with an additional band of similar molecular weight observed in rat brain tissue, consistent with Glial fibrillary acidic protein / GFAP, a major astrocytic intermediate filament protein involved in maintenance of neural cytoskeletal organization. The stacked banding pattern in the human brain sample likely reflects a combination of GFAP isoforms and partial proteolytic processing products commonly observed in neural tissue extracts.