GFAP Antibody / Glial Fibrillary Acidic Protein [clone GFAP/8616R] (V4513)

Western blot testing of human brain tissue with GFAP antibody. Predicted molecular weight ~50 kDa.

SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GFAP/8616R) as confirmation of integrity and purity.

IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/8616R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

GFAP Antibody Multi-Tissue WB. Western blot analysis of human brain, heart, intestine, kidney, liver, lung, ovary, pancreas, prostate, skeletal muscle, skin, spleen, stomach, and testis tissue lysates using GFAP Antibody clone GFAP/8616R. A distinct band is detected at approximately 50 kDa selectively in human brain lysate, consistent with the predicted molecular weight of Glial fibrillary acidic protein / GFAP, an astrocyte-associated intermediate filament protein enriched within central nervous system tissue. Subtle lower molecular weight signal broadening beneath the primary band likely reflects GFAP isoforms and/or partial proteolytic processing products commonly observed in neural tissue extracts.

GFAP Antibody Brain Tissue WB. Western blot analysis of human brain, mouse brain, rat brain, hamster brain, and guinea pig brain tissue lysates using GFAP Antibody clone GFAP/8616R. A strong band is detected at approximately 50 kDa in human brain lysate, with an additional band of similar molecular weight observed in guinea pig brain tissue, consistent with the predicted molecular weight of Glial fibrillary acidic protein / GFAP, a major astrocytic intermediate filament protein involved in maintenance of neural cytoskeletal organization and central nervous system integrity. Multiple faint lower molecular weight bands forming a subtle stacked pattern beneath the primary human brain signal likely represent GFAP isoforms and/or partial proteolytic processing products commonly observed in neural tissue extracts.