GFAP Antibody / Glial Fibrillary Acidic Protein [clone GFAP/6886] (V4525)

IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/6886). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/6886). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human brain tissue lysate with GFAP antibody (clone GFAP/6886).

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using GFAP antibody (GFAP/6886). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GFAP/6886) as confirmation of integrity and purity.

GFAP Antibody Brain Tissue WB. Western blot analysis of human brain, mouse brain, rat brain, guinea pig brain, and hamster brain tissue lysates using GFAP Antibody clone GFAP/6886. A strong band is detected at approximately 50 kDa in human brain lysate, with additional bands of similar molecular weight observed in rat and guinea pig brain samples, consistent with the predicted molecular weight of Glial fibrillary acidic protein / GFAP, an astrocytic intermediate filament protein involved in maintenance of neural cytoskeletal structure and central nervous system integrity. Subtle lower molecular weight signal broadening beneath the primary human brain band likely reflects GFAP isoforms and/or partial proteolytic processing products commonly observed in brain tissue extracts.