GFAP Antibody / Glial Fibrillary Acidic Protein [clone GFAP/6880] (V4018)

IHC staining of FFPE human cerebellum tissue with GFAP antibody (clone GFAP/6880). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cerebral cortex tissue with GFAP antibody (clone GFAP/6880). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC analysis of FFPE human heart tissue using GFAP antibody (clone GFAP/6880). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using GFAP antibody (clone GFAP/6880). These results demonstrate the foremost specificity of the GFAP/6880 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Western blot testing of human brain tissue with GFAP antibody. Predicted molecular weight ~50 kDa.

SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GFAP/6880) as confirmation of integrity and purity.

GFAP Antibody Brain Tissue WB. Western blot analysis of human brain, mouse brain, rat brain, hamster brain, guinea pig brain, and rabbit brain tissue lysates using GFAP Antibody clone GFAP/6880. Distinct bands are detected at approximately 50 kDa in human and rabbit brain lysates, consistent with the predicted molecular weight of Glial fibrillary acidic protein / GFAP, a major astrocytic intermediate filament protein involved in maintenance of neural cytoskeletal architecture. Multiple closely migrating lower molecular weight bands observed in the human brain sample likely represent GFAP isoforms and/or partial proteolytic processing products commonly encountered in brain tissue lysates.